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KMID : 0624620090420020106
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BMB Reports
2009 Volume.42 No. 2 p.106 ~ p.112
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The bio-complex “reaction pattern in vertebrate cells” reduces cytokine-induced cellular adhesion molecule mRNA expression in human endothelial cells by attenuation of NF-kappaB translocation
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Ronnau Cindy
Herbert Liebermann E. H.
Helbig Franz
Staudt Alexander
Stephan Felix B.
Ewert Ralf
Landsberger Martin
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Abstract
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The bio-complex “reaction pattern in vertebrate cells” (RiV) is mainly represented by characteristic exosome-like particles -- probably as reaction products of cells to specific stress. The transcription factor NF-kappaB plays a central role in inflammation. We tested the hypothesis that RiV particle preparations (RiV-PP) reduce cellular adhesion molecule (CAM) expression (ICAM-1, VCAM-1, E-selectin) by the attenuation of NF-kappaB translocation in human umbilical vein endothelial cells (HUVEC). After 4 hours, pre-incubation of HUVEC with RiV-PP before stimulation with TNF-alpha significantly reduced ICAM-1 (65.5±10.3%) and VCAM-1 (71.1±12.3%) mRNA expression compared to TNF-alpha-treated cells (100%, n=7). ICAM-1 surface expression was significantly albeit marginally reduced in RiV/TNF-alpha- treated cells (92.0±5.6%, n=4). No significant effect was observed on VCAM-1 surface expression. In RiV/TNF-alpha-treated cells (n=4), NF-kappaB subunits p50 (85.7±4.1%) and p65 (85.0±1.8%) nuclear translocation was significantly reduced. RiV-PP may exert an anti-inflammatory effect in HUVEC by reducing CAM mRNA expression via attenuation of p50 and p65 translocation.
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KEYWORD
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Endothelial function, E-selectin, ICAM-1, Inflammation, VCAM-1
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